Improvement of Glucoamylase Production by Aspergillus awamori Using Microbial Biotechnology Techniques
نویسندگان
چکیده
منابع مشابه
Production and characterization of glucoamylase from fungus Aspergillus awamori expressed in yeast Saccharomyces cerevisiae using different carbon sources
Glucoamylase is widely used in the food industry to produce high glucose syrup, and also in fermentation processes for production beer and ethanol. In this work the productivity of the glucoamylase of Aspergillus awamori expressed by the yeast Saccharomyces cerevisiae, produced in submerged fermentation using different starches, was evaluated and characterized physico-chemically. The enzyme pre...
متن کاملExtraction and Purification of Glucoamylase and Protease Produced by Aspergillus awamori in a Single-Stage Fermentation
Simultaneous extraction and purification of glucoamylase and protease produced concomitantly by Aspergillus awamori Nakazawa MTCC 6652 in a single fermentor using solid-state fermentation (SSF) has been studied. Soaking for 2 h at room temperature (around 30 °C) in 10 % glycerol was found to be most suitable for optimum simultaneous extraction of glucoamylase and protease with the yield of 8645...
متن کاملProduction of Glucoamylase by Aspergillus Niger under Solid State Fermentation
In this study, Glucoamylase production by Aspergillus niger was investigated under solid state fermentation using low cost by-products of agricultural processes as substrate. Highest enzyme production was observed when a combination of wheat bran (WB) and corn flour (CF) was used as compared to WB+ rice bran, WB+ rice flour and WB alone. Several compositions of corn flours were experimented. Th...
متن کاملTHE PRODUCTION OF GLUCOAMYLASE BY ASPERGILLUS NIGER UNDER SOLID STATE CONDITIONS (RESEARCH NOTE)
In this study, Glucoamylase production by Aspergillus Niger was investigated under solid state conditions with low cost by-products of agricultural processes as substrate. Highest enzyme production was observed when a combination of wheat bran (WB) and corn flour (CF) was used as compared to WB+ rice bran, WB+ rice flour and WB alone. Different additions of (CF) were tested and WB+ 10% CF showe...
متن کاملProtein engineering of Aspergillus awamori glucoamylase to increase its pH optimum.
To increase the pH optimum of glucoamylase (GA), five mutations-S411G, S411A, S411C, S411H and S411D--were designed to destabilize the carboxylate ion form of Glu400, the catalytic base, by removing or weakening the hydrogen bond between Ser411 and Glu400, and thereby raising its pK. The substitution of alanine, histidine and aspartate were also designed to study the additional effects of polar...
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ژورنال
عنوان ژورنال: Biotechnology(Faisalabad)
سال: 2008
ISSN: 1682-296X
DOI: 10.3923/biotech.2008.456.462